Rafted Type 1 and Type 2 EBV infected NOKs have similar levels of lytic EBV reactivation.

Two lines each of Akata EBV-infected or AG876 EBV-infected cells were differentiated on raft cultures (each in the context of the “NOKS-2 line”). (A) Histology was performed on rafted cells and sections were stained with BZLF1 antibody and counter-stained with hematoxylin (choosing regions of the raft that had some level of K10 expression). Representative sections are shown for NOKs infected with Akata EBV or AG876 EBV. BZLF1-expressing cells are indicated by arrows. (B) In Situ hybridization was performed to detect EBV EBERs. Representative sections are shown for uninfected NOKs or NOKs infected with AG876 EBV.