NOKs infected with Type 1 and type 2 EBV exhibit similar levels of lytic reactivation in response to differentiation stimuli.

(A) Uninfected NOKs, two different lines of AG876 EBV-infected NOKs cells, and four different lines of Akata EBV-infected NOKs (each in the context of the “NOKs-2” line) were grown in sub-confluent conditions in KSFM medium without supplements for 24 hours. Cells were then treated with TPA at 20ng/mL for 48 hours, and immunoblot analysis was performed to assess expression of BZLF1, BRLF1, LMP1, BMRF1 or tubulin (loading control) as indicated. (B) Akata EBV-infected, AG876-EBV infected or uninfected NOKs were differentiated by suspending the cells in 1.6% methylcellulose containing KSFM medium without supplements for 72 hours, and then immunoblot analysis was performed to assess expression of the BZLF1, BRLF1, LMP1, BMRF1 and tubulin (loading control) proteins.