A. Representative fluorescence microscopy images of TUNEL staining of HIO and PMN-HIO histology sections. HIOs were microinjected with STM and either cultured alone or co-cultured with PMNs in the presence of inhibitors for Caspase-1 (z-YVAD), Caspase-3 (z-DEVD), or DMSO control. B. Quantitation of the percent of lumen filled with TUNEL-positive cells of STM-infected HIOs or PMN-HIOs with indicated treatments. C. Quantitation of the percent of infected cells per HIO based on 3 fields per view per HIO. D. Quantitation of number of bacteria per infected cell in PMN-HIOs based on 3 fields per view per HIO. E. Quantitation of epithelium associated bacteria. Number of bacteria within 50μm of the apical epithelial surface were counted and normali...
<p>(A) Media alone or <i>S</i>. <i>pneumoniae-</i>containing media was added to the lower chamber of...
<p>Control MCF10A-pLXSN and MCF10A-TSPO cells were seeded and cultured in Matrigel. On day 20, cultu...
Cted cells were cultured further. The number of viable bacteria in each well was determined by the C...
A. Representative fluorescence microscopy images of TUNEL staining of PMN-HIO frozen histology secti...
A. Transmigration of PMNs into the lumen of HIOs was quantified using flow cytometry. HIOs were micr...
A. Immunofluorescent images of TUNEL staining of histology sections of HIOs and PMN-HIOs injected wi...
A. Cartoon model showing Caspase-1 activation in PMNs leading to IL-1β production which regulates ce...
<p><b>(A-D)</b> iHIOs microinjected with saline or 10<sup>3</sup> commensal bacteria or O157:H7 in t...
NHBE cells were exposed for 3 hours to media only (control), 20 μg/ml LL-37 only, PAO1/ heat killed ...
<p>S2 cells were infected with <i>Pa. acanthamoebae</i> (MOI 5) and were incubated for the indicated...
<p>(A) BMDM were infected with <i>L. monocytogenes</i> and gentamicin (480 Da) was added 30 min pi t...
<p>S2 cells were infected with <i>S. negevensis</i> (MOI 5) and were incubated for the indicated tim...
<p>Phaygocytosis (A), as measured by uptake of GFP<sup>+</sup><i>P. aeruginosa</i> 19660 by PMN, was...
<p>Monolayers of murine oviduct epithelial cells were infected with <i>C</i>. <i>trachomatis</i> L a...
(A) Neutrophils were left uninfected (NS) or were infected with KPn (MOI 10). The level of PS was mo...
<p>(A) Media alone or <i>S</i>. <i>pneumoniae-</i>containing media was added to the lower chamber of...
<p>Control MCF10A-pLXSN and MCF10A-TSPO cells were seeded and cultured in Matrigel. On day 20, cultu...
Cted cells were cultured further. The number of viable bacteria in each well was determined by the C...
A. Representative fluorescence microscopy images of TUNEL staining of PMN-HIO frozen histology secti...
A. Transmigration of PMNs into the lumen of HIOs was quantified using flow cytometry. HIOs were micr...
A. Immunofluorescent images of TUNEL staining of histology sections of HIOs and PMN-HIOs injected wi...
A. Cartoon model showing Caspase-1 activation in PMNs leading to IL-1β production which regulates ce...
<p><b>(A-D)</b> iHIOs microinjected with saline or 10<sup>3</sup> commensal bacteria or O157:H7 in t...
NHBE cells were exposed for 3 hours to media only (control), 20 μg/ml LL-37 only, PAO1/ heat killed ...
<p>S2 cells were infected with <i>Pa. acanthamoebae</i> (MOI 5) and were incubated for the indicated...
<p>(A) BMDM were infected with <i>L. monocytogenes</i> and gentamicin (480 Da) was added 30 min pi t...
<p>S2 cells were infected with <i>S. negevensis</i> (MOI 5) and were incubated for the indicated tim...
<p>Phaygocytosis (A), as measured by uptake of GFP<sup>+</sup><i>P. aeruginosa</i> 19660 by PMN, was...
<p>Monolayers of murine oviduct epithelial cells were infected with <i>C</i>. <i>trachomatis</i> L a...
(A) Neutrophils were left uninfected (NS) or were infected with KPn (MOI 10). The level of PS was mo...
<p>(A) Media alone or <i>S</i>. <i>pneumoniae-</i>containing media was added to the lower chamber of...
<p>Control MCF10A-pLXSN and MCF10A-TSPO cells were seeded and cultured in Matrigel. On day 20, cultu...
Cted cells were cultured further. The number of viable bacteria in each well was determined by the C...