Effect of mouse strain and coagulation factors on liver transduction by species C Ad vectors.

Mice were injected IV with luciferase-expressing HD-Ad vectors at a dose of 4x1011 vp/kg. Mice were euthanized 48 h later, and liver luciferase activity was measured. Liver luciferase activity is shown in (A) BALB/c mice and (B) C57BL/6 mice. (C) BALB/c mice or (D) C57BL/6 mice were pre-treated with warfarin to deplete coagulation factors, then injected with HD-Ad5 or HD-Ad6 vectors and evaluated for liver luciferase activity. Control mice were pre-treated with vehicle (oil) before injection with vectors. n = 4–7 mice per group, mean ± SD. Statistical comparisons were performed using analysis of variance (ANOVA) and the Holm-Sidak post hoc test. Groups that do not share the same letter are significantly different from each other (P ≤ 0.05).