Hepatic luciferase expression is reduced with both KKTK mutation and warfarinization.

<p>A–F) Nude NMRI mice carrying mammary fat pad tumors were treated intravenously with 4×10¹⁰ VP of Ad5/3luc1, Ad5/3lucS* or Ad5luc1. Some groups were pretreated with warfarin to deplete coagulation factors. 48 hours after virus injection mice were sacrificed and luciferase expression in homogenized tissue lysates was quantified and normalized for total protein content of lysates. KKTK mutation reduced hepatic transgene expression, but reduction was also seen in tumors and other tissues. Mean+SD, n = 3–5 mice per group. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001 vs. Ad5/3luc1.