The use of DNA markers for genetic differentiation of common (Avena sativa L.) and naked oat (Avena nuda L.)

Uporabnost genetske identifikacije sort se kaže pri analizah kakovosti semena, ko se na osnovi morfoloških znakov semena pri analizi čistote in/ali klic v postopku kalivosti pojavi sum na drugo sorto, vrsto ali celo rod. Z aplikativno uporabo genetskih analiz smo uspešno rešili dvom o pristnosti sorte \u27Kamil\u27 vrste golega ovsa (Avena nuda L.). Med semenom omenjene sorte je bilo namreč prisotno tudi seme s plevo, a se ni dalo z gotovostjo potrditi ali gre za seme iste vrste s plevo ali za primes semen navadnega ovsa (Avena sativa L.). Na podlagi fenotipa semena sorte \u27Kamil\u27 (dva neodvisna vzorca 284 in 285) so bili pripravljeni štirje pod-vzorci (284AN, 284AS, 285AN, 285AS), kjer AN pomeni golce, AS pa plevence. Kot standardi navadnega ovsa so bili v genetsko identifikacijo vključene še sorta navadnega ovsa \u27Noni\u27 in štiri akcesije navadnega ovsa (ACC378, ACC379, ACC380, ACC381) iz Slovenske rastlinske genske banke. Skupno je bilo v genotipizacijo vključenih 36 vzorcev rastlin rodu Avena sp. Z uporabo seta šestih visoko informativnih SSR markerjev in rezultatov analize parametrov raznolikosti na posameznih lokusih ter parnih genetskih primerjav smo lahko potrdili, da so analizirani pod-vzorci pripadali isti vrsti in sorti, t.j. golemu ovsu \u27Kamil\u27. Poleg tega so bili kljub morfološki podobnosti plevenci sorte Kamil‘ genetsko dovolj različni od analizirane sorte in/ali akcesij navadnega ovsa.The usefulness of genetic identification of varieties for seed quality analyses becomes important, when we suspect the presence of another variety, species or even genus, based on morphological seed traits in the purity analysis and germination test, With genetic analyses, the doubt about the authenticity of the naked oats (Avena nuda L.) variety \u27Kamil\u27 was successfully solved. There were atypical seeds with chaff among the samples of this variety, so it was not possible to confirm with certainty, whether these were seeds of the same species/variety or impurities of common oats (Avena sativa L.). Based on the seed phenotype of the \u27Kamil\u27 variety (two independent samples 284 and 285), four sub-samples were prepared (284AN, 284AS, 285AN, 285AS); AN label was the sub-sample with naked seeds and AS label was the sub-sample with chaffed seeds. In addition, another variety (\u27Noni\u27) and four accessions of common oats (ACC378, ACC379, ACC380, ACC381) from the Slovene Plant Gene Bank were included as common oat standards. A total of 36 plant samples of Avena sp. were included in the genetic differentiation. Using a set of six highly informative SSR markers and the results of diversity parameters at individual loci and paired genetic comparisons, we were able to confirm that the analysed sub-samples belonged to the same variety, i.e. \u27Kamil\u27. In addition, despite the morphological similarity, the chaffed seeds of naked oats variety ‘Kamil’ were sufficiently (genetically) different from the analysed variety and/or accessions of common oats