Direct sequencing of the IC amplified from bisulfite-treated samples containing 106 IC copies mixed with 500 ng of DNA (A) or 108 IC copies mixed with either 500 ng (B) or 5 ng (C) of genomic DNA. Direct sequencing of the LINE-1 sequence amplified from bisulfite-treated samples containing 106 IC copies and 5 ng (D), 50 ng (E) and 500 ng (F) of genomic DNA. Unconverted cytosines were indicated by arrows. (DOCX)</p
<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using n...
<p>(A) Shown is a schematic representation of RNA molecules; colored boxes represent single bases. R...
During bisulfite genomic sequencing projects large amount of data are generated. The Bisulfite seque...
<p>DNA was amplified and sequenced using each primer set on an ABI automated sequencer with dye term...
<p>DNA was bisulfite converted using nine different commercially available kits. Each kit was proces...
After bisulfite treatment, (A) the internal control (IC) used to monitor bisulfite conversion effici...
<p>Ratios of brCs were determined by bisulfite pyrosequencing (Mean±SD, triplicated assays). (A) The...
The bisulfite genomic sequencing (BGS) proto-col has gained worldwide popularity as the me-thod of c...
<p><b>A</b>. Intact LINE-1 is approximately 6 kb long, and comprised of 5′-untranslated region (5′UT...
Motivation: Cytosine DNA methylation is one of the major epigenetic modifications and influences gen...
Bisulfite genomic sequencing is a widely used technique for analyzing cytosine-methylation of DNA. B...
DNA methylation is an essential epigenetic modification in the human genome. For the investigation o...
<p>Agarose gel electrophoresis of genomic and bisulfite-converted DNA (2 μg each). The bisulfite con...
<p>Left panel depicts DNA methylation values (Beta) from ICF and healthy donors using both methodolo...
During bisulfite genomic sequencing projects large amount of data are generated. The Bisulfite seque...
<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using n...
<p>(A) Shown is a schematic representation of RNA molecules; colored boxes represent single bases. R...
During bisulfite genomic sequencing projects large amount of data are generated. The Bisulfite seque...
<p>DNA was amplified and sequenced using each primer set on an ABI automated sequencer with dye term...
<p>DNA was bisulfite converted using nine different commercially available kits. Each kit was proces...
After bisulfite treatment, (A) the internal control (IC) used to monitor bisulfite conversion effici...
<p>Ratios of brCs were determined by bisulfite pyrosequencing (Mean±SD, triplicated assays). (A) The...
The bisulfite genomic sequencing (BGS) proto-col has gained worldwide popularity as the me-thod of c...
<p><b>A</b>. Intact LINE-1 is approximately 6 kb long, and comprised of 5′-untranslated region (5′UT...
Motivation: Cytosine DNA methylation is one of the major epigenetic modifications and influences gen...
Bisulfite genomic sequencing is a widely used technique for analyzing cytosine-methylation of DNA. B...
DNA methylation is an essential epigenetic modification in the human genome. For the investigation o...
<p>Agarose gel electrophoresis of genomic and bisulfite-converted DNA (2 μg each). The bisulfite con...
<p>Left panel depicts DNA methylation values (Beta) from ICF and healthy donors using both methodolo...
During bisulfite genomic sequencing projects large amount of data are generated. The Bisulfite seque...
<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using n...
<p>(A) Shown is a schematic representation of RNA molecules; colored boxes represent single bases. R...
During bisulfite genomic sequencing projects large amount of data are generated. The Bisulfite seque...