DNA Yield and Purity after Bisulfite Conversion Applying Nine Different Kits.

<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using nine different commercially available kits. Each bisulfite conversion was conducted in nine replicates for each kit. <b>Left panel:</b> UV spectra of bisulfite-converted and purified DNA (<b>left panel</b>). <b>Middle and right panel:</b> DNA yield of bisulfite-converted DNA as determined via UV (<b>middle panel</b>) measurement and via CFF qPCR assay (<b>right panel</b>), respectively. Shown are mean values of triplicate PCR measurements.