<p>DNA was bisulfite converted using nine different commercially available kits. Each kit was processed in nine independent bisulfite reactions using 2 μg HMW input DNA, each. The specific conversion of cytosines to uracils at two different genetic loci were analyzed by means of the CFP clone sequencing assay.</p
Bisulfite (BS) conversion, which includes a series of chemical reactions using bisulfite, is a prere...
Bisulfite genomic sequencing is a widely used technique for analyzing cytosine-methylation of DNA. B...
<p>Ratios of brCs were determined by bisulfite pyrosequencing (Mean±SD, triplicated assays). (A) The...
<p>Agarose gel electrophoresis of genomic and bisulfite-converted DNA (2 μg each). The bisulfite con...
<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using n...
After bisulfite treatment, (A) the internal control (IC) used to monitor bisulfite conversion effici...
<p>The conversion efficiency of the unmethylated cytosines to thymines was calculated per kit. All t...
<p><b>A:</b> Basic principle of the CFP clone sequencing assay for quantifying the bisulfite convers...
<p>DNA was amplified and sequenced using each primer set on an ABI automated sequencer with dye term...
<p>Total yield of bisulfite-converted DNA derived from the direct processing of 10 μm FFPE placental...
Direct sequencing of the IC amplified from bisulfite-treated samples containing 106 IC copies mixed ...
DNA methylation is an essential epigenetic modification in the human genome. For the investigation o...
Introduction—Bisulfite treatment of DNA introduces methylation-dependent sequence changes through se...
DNA methylation is the most studied epigenetic modification with a wide range of regulatory function...
DNA methylation analyses usually require a preceding bisulfite conversion of the DNA. The choice of ...
Bisulfite (BS) conversion, which includes a series of chemical reactions using bisulfite, is a prere...
Bisulfite genomic sequencing is a widely used technique for analyzing cytosine-methylation of DNA. B...
<p>Ratios of brCs were determined by bisulfite pyrosequencing (Mean±SD, triplicated assays). (A) The...
<p>Agarose gel electrophoresis of genomic and bisulfite-converted DNA (2 μg each). The bisulfite con...
<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using n...
After bisulfite treatment, (A) the internal control (IC) used to monitor bisulfite conversion effici...
<p>The conversion efficiency of the unmethylated cytosines to thymines was calculated per kit. All t...
<p><b>A:</b> Basic principle of the CFP clone sequencing assay for quantifying the bisulfite convers...
<p>DNA was amplified and sequenced using each primer set on an ABI automated sequencer with dye term...
<p>Total yield of bisulfite-converted DNA derived from the direct processing of 10 μm FFPE placental...
Direct sequencing of the IC amplified from bisulfite-treated samples containing 106 IC copies mixed ...
DNA methylation is an essential epigenetic modification in the human genome. For the investigation o...
Introduction—Bisulfite treatment of DNA introduces methylation-dependent sequence changes through se...
DNA methylation is the most studied epigenetic modification with a wide range of regulatory function...
DNA methylation analyses usually require a preceding bisulfite conversion of the DNA. The choice of ...
Bisulfite (BS) conversion, which includes a series of chemical reactions using bisulfite, is a prere...
Bisulfite genomic sequencing is a widely used technique for analyzing cytosine-methylation of DNA. B...
<p>Ratios of brCs were determined by bisulfite pyrosequencing (Mean±SD, triplicated assays). (A) The...