<p>Total yield of bisulfite-converted DNA derived from the direct processing of 10 μm FFPE placental tissue sections using three bisulfite conversion kits. Each kit was tested in 9 replicates. Shown are mean values (± standard deviation) of triplicate CFF qPCR measurements (<b>B</b>) and results from a single UV measurement (<b>A</b>), respectively.</p
<p>*Starting material: extracted DNA.</p>†‡<p>These kits share the identical bisulfite conversion co...
<p>DNA was extracted from 27 FFPE samples of normal breast, normal liver and tumorous tongue and the...
In most pathology laboratories worldwide, formalin-fixed paraffin embedded (FFPE) samples are the on...
<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using n...
<p>DNA was bisulfite converted using nine different commercially available kits. Each kit was proces...
<p>Agarose gel electrophoresis of genomic and bisulfite-converted DNA (2 μg each). The bisulfite con...
<p>(A) qPCR–amplification of different amounts (10–3,840 ng) of bisulfite-converted template DNA fro...
<p>Quantitative real-time PCR analysis of a dilution series of genomic (unconverted) and bisulfite-c...
<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using n...
Robust standardized methods to extract DNA from formalin-fixed paraffin-embedded tissues are require...
<p><b>A:</b> Inhibitory effect of eluate derived from different bisulfite conversion kits. Water was...
DNA methylation analyses usually require a preceding bisulfite conversion of the DNA. The choice of ...
<p>As a quality control step, the bisulfite converted DNA was amplified with regular non-bisulfite s...
Abstract Background Formalin fixed paraffin embedded (FFPE) tumor samples are a major source of DNA ...
Additional file 1: Figure. An example of a ddPCR assay for absolute quantification of DNA copies. Th...
<p>*Starting material: extracted DNA.</p>†‡<p>These kits share the identical bisulfite conversion co...
<p>DNA was extracted from 27 FFPE samples of normal breast, normal liver and tumorous tongue and the...
In most pathology laboratories worldwide, formalin-fixed paraffin embedded (FFPE) samples are the on...
<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using n...
<p>DNA was bisulfite converted using nine different commercially available kits. Each kit was proces...
<p>Agarose gel electrophoresis of genomic and bisulfite-converted DNA (2 μg each). The bisulfite con...
<p>(A) qPCR–amplification of different amounts (10–3,840 ng) of bisulfite-converted template DNA fro...
<p>Quantitative real-time PCR analysis of a dilution series of genomic (unconverted) and bisulfite-c...
<p>HMW (<b>upper panel</b>) and FFPE tissue (<b>lower panel</b>) DNA was bisulfite-converted using n...
Robust standardized methods to extract DNA from formalin-fixed paraffin-embedded tissues are require...
<p><b>A:</b> Inhibitory effect of eluate derived from different bisulfite conversion kits. Water was...
DNA methylation analyses usually require a preceding bisulfite conversion of the DNA. The choice of ...
<p>As a quality control step, the bisulfite converted DNA was amplified with regular non-bisulfite s...
Abstract Background Formalin fixed paraffin embedded (FFPE) tumor samples are a major source of DNA ...
Additional file 1: Figure. An example of a ddPCR assay for absolute quantification of DNA copies. Th...
<p>*Starting material: extracted DNA.</p>†‡<p>These kits share the identical bisulfite conversion co...
<p>DNA was extracted from 27 FFPE samples of normal breast, normal liver and tumorous tongue and the...
In most pathology laboratories worldwide, formalin-fixed paraffin embedded (FFPE) samples are the on...