Early adulthood metabolic changes are reduced in the absence of <i>p21</i>.

(A) qPCR of cell cycle inhibitors in epididymal fat in 6 months old wild type (WT) and Tph1 deficient (Tph1-/-) mice. (B) Quantification of body weight of WT, Tph1-/-, p21 deficient (p21-/-) and Tph1, p21 double deficient (Tph1-/-;p21-/-) mice at 2 and 6 months of age. (C) Haematoxylin and Eosin (H&E) staining of epididymal fat in WT, Tph1-/-, p21-/- and Tph1-/-;p21-/- mice at 6 months of age. (D) Quantification of adipocyte size in epididymal fat in 6 months old mice. (E) Quantification of blood cholesterol (Chol) and triglyceride (TG) levels in WT, Tph1-/-, p21-/- and Tph1-/-;p21-/- mice at 6 months of age. (F) Blood glucose levels in WT, Tph1-/-, p21-/- and Tph1-/-;p21-/- mice at 6 months of age in fasted and fed state. (G) Glucose tolerance test (GTT) in WT, Tph1-/-, p21-/- and Tph1-/-;p21-/- mice at 6 months of age. (H) Area under the curve (AUC) of GTT in WT, Tph1-/-, p21-/- and Tph1-/-;p21-/- mice at 6 months of age.Results are average ± SEM (n≥5), *p < 0.05.