Early adulthood metabolic changes are reduced in the absence of <i>Tph1</i>.

(A) qPCR of serotonin synthetic enzyme (Tph1), serotonin transporter (Sert), serotonin receptors (5-Ht1b, 5-Ht2a, 5-Ht2b) in epididymal fat in 2 and 6 months old mice. (B) Body weight of wild type (WT) and Tph1 deficient (Tph1-/-) mice at 2 and 6 months of age. (C) Haematoxylin and Eosin (H&E) staining of epididymal fat in WT and Tph1-/- mice at 6 months of age. (D) Quantification of adipocyte size in epididymal fat in 6 months old mice. (E) qPCR of macrophage marker F480 and inflammatory mediators in epididymal fat in 6 months old mice. (F) Blood glucose quantification in 2 and 6 months old mice measured in overnight fasted and fed state. (G) Glucose tolerance test (GTT) and area under the curve (AUC) quantification in 6 months old mice. Results are average ± SEM (n≥5), *p < 0.05.