Complementary DNA probe for the detection of isolates of Indian peanut clump virus

The main aim of the present study was to use the clones of PCV genome and select a clone which would hybridize with RNA of several PCV isolates which could be used in the preparation of a C-DNA probe. For this purpose Northern Dot Blot hybridization technique was used which was probed with nick translated 32 p. labeled C-DNA clones of H-IPCV. Among the H-IPCV clines analyzed the 2 kbp and 3.5-4 kbp clines were employed in this study to probe the IPCV isolates. Autoradiographic signals showed greater hybridization could be achieved with 3.5-4 kbp clone than 2 kbp clone using the pure virus and nucleic acid of H-IPCV and with RNA of partially purified preparations of L-IPCV and total nucleic acids of B-IPCV isolate