The nucleotide sequence of RNA-1 of Indian peanut clump furovius

The nucleotide sequence of RNA-1 of an isolate of the H serotype of Indian peanut clump virus (IPCV-H) was shown to comprise 5 841 nucleotides. The RNA contains three open reading frames (ORF) which are between nucleo¬tides 133 and 3522, nucleotides 3526 and 5103 (assuming expression by suppression of the ORF 1 termination codon) and nucleotides 5 168 and 5 539. The encoded polypeptides have M,, of 129687 (pl30), 60188 (p60) and 14281 (pl4). ORF 2 is thought to be expressed by suppression of the termination codon of ORF 1 to produce a M,. 189975 product (pl90). pl30 contains sequences characteristic of proteins with methyl transferase and NTP-binding properties and pl90 contains these and sequences characteristic of RNA-dependent RNA polymerases. The nucleotide sequence of IPCV RNA-1 is similar to that of peanut clump virus (PCV) and corresponding encoded polypeptides are 88% (pl30), 95% p60 and 75% (pl4) identical. The sequences of the translation products are also similar to those of soil-borne wheat mosaic virus and barley stripe mosaic virus. Oligonucleotide primers, designed on the basis of the sequences of RNA-1 of IPCV and PCV, were effective in reverse transcription-PCR amplification of these RNAs and that of IPCV isolates of the serologically distinct L and T serotypes