Indian Peanut Clump Virus Isolates: Host Range, Symptomatology, Serological Relationships, and Some Physical Properties

The symptomatology of Indian peanut clump virus (IPCV) isolates collected from five different geographical locations, Bapatla (B), Chinnaganjam (C), Hyderabad (H), Ludhiana (L), and Talod (T), differed. B-IPCV and C-IPCV were indistinguishable by host range but could be distinguished from the other isolates by symptoms on Canavalia ensiformis, Nicotiana clevelandii × glutinosa, Phaseolus vulgaris, and Vigna unguiculata. B-IPCV, C-IPCV, and T-IPCV were related serologically, but could be distinguished from H-IPCV and L-IPCV isolates in serological tests. The five isolates could not be distinguished on the basis of particle size. Each isolate contained two RNA species of 1.90 × 106 and 1.65 × 106 Mr estimated under nondenaturing conditions and a single polypeptide of 24 × 103 Mr. Significance of these findings for the diagnosis of IPCV and for screening of peanut genotypes for resistance is discussed