PRODUCTION AND CHARACTERIZATION OF ANTI FIM-C Salmonella typhi NATIVE PROTEIN ANTIBODY IN DDY MICE

Typhoid fever is a disease affect many people in developing countries including Indonesia. Prevention can be aided by vaccination to the bacteria; however it still needs further research. This research is aimed to determine the immunogenicity of fim-C native protein Salmonella typhi in vivo as a vaccine candidate in ddY mice. Experimental method is used in this research. This research used antigen of pure fim-C native recombinant protein from its previous research. The ddY mice are categorized into five groups, namely experiment group 1 (protein Fim-C+Freund's Complete/Incomplete Adjuvant), experiment group 2 (protein Fim-C S. typhi), control group 1 (FCA/FIA adjuvants), control group 2 (PBS1x), and normal group (PBS1x). The results showed that the ddY mice produced antibody response after subcutaneous injection of the Fim-C S. typhi native protein +adjuvant or without adjuvant. The antibody responses with Fim-C S. typhi native protein as antigen+adjuvant gave higher absorbans than the Fim-C antigen without adjuvant. It is also showed that the antibody titer from the first to fourth injection has gradually increased. These data showed that Fim-C S. Typhi native protein had higher immunogenicity. It is concluded that the Fim-C S. typhi native protein can be used as a potential recombinant vaccine candidate against typhoid fever