BIOLOGICAL EFFECTS OF ULTRASONIC RADIATION ON Chaetoceros muelleri

设计了一个超声频率、超声功率和辐射时间3因素4水平的正交实验 ,研究超声辐射对牟氏角毛藻的生物效应。实验结果表明 ,超声频率对牟氏角毛藻的生物效应显著 ,而超声功率和辐射时间的影响相对较小。在正交实验所确定的最佳超声辐射条件下 ,牟氏角毛藻生长速率常数最高可达0.348d-1,是对照组的1.78倍 ,其脂肪酸不饱和度最高可达68.4 %,比对照组提高5.2%,主要不饱和脂肪酸的百分含量均可得到不同程度的提高。A three-factor and four-level orthogonal experiment, composed of ultrasonic frequency, power and radiative duration, was designed to study the biological effect of ultrasonic radiation on Chaetoceros mulleri. The experimental results suggested that ultrasonic frequency had significant biological effect, and radiative duration and ultrasonic power had less influence on the alga. The maximum constant of growth rate of C. mulleri could be 0.348 d-1 which was greater than that of the controlled by a factor of 1.78, the maximum unsaturation could be 68.4%,an increase of 5.2% as compared with that of the controlled, and the percentages of major unsaturated fatty acids in TFA could increase more or less under the optimum ultrasonic condition deduced by the orthogonal experiment.国家自然科学基金资助项目39870565号;; 福建省自然科学基金资助项目C97006