Study on the Effects of Ultrasonic Wave on Hatching of Fertilized Egg in Four Marine Fish

采用复合式纵向压电换能器 ,谐振频率为 2 1.5k Hz,谐振阻抗为 12 0Ω ,施加电压值为 80 V的超声波辐照海水鱼类受精卵 ,研究不同的超声辐射时间和辐射次数对大弹涂鱼、中华乌塘鳢、真鲷和大黄鱼受精卵孵化的影响 .结果表明 ,超声处理大弹涂鱼胚胎发育后期的受精卵 ,可提高其孵化率 ,但对大弹涂鱼和中华乌塘鳢胚胎发育早期受精卵的孵化有负面影响 .每次辐射时间为 1min、总辐射次数为 4次的辐射剂量有利于真鲷受精卵的孵化 ;每次辐射时间超过 1min,孵化率降低 .以每次辐射时间为 10 s或 30 s处理大黄鱼受精卵 ,其孵化率分别比对照组高出 10 %和 12 %Effects of fixed frequency and intensity ultrasonic waves, with different radiation duration and times, on hatching of fertilized egg in Boleophthalmus pectinirostris (Linnaeus), Bostrichthys sinensis (Lacepede), Pagrosomus major (Temminck et Schlegel) and Pseudosciaena crocea (Richardson) were investigated. The results showed that ultrasonic radiation increased hatching rates of Boleophthalmus pectinirostris fertilized eggs for late developmental embryos, but it had negative effects on the hatching of Boleophthalmus pectinirostris and Bostrichthys sinensis fertilized eggs for early developmental embryos. Ultrasonic radiation, in 1 minute each time and 4 times total, was favorable to the hatching of Pagrosomus major fertilized eggs, whereas the hatching rates decreased if the radiation treatment duration was over 1 minute each time. Hatching rates of Pseudosciaena crocea fertilized eggs, treated with ultrasonic radiation in 10 s or 30 s each time, were 10% or 12% higher than that of the control.福建省自然科学基金资助项目!( B9910 0 0 6,B0 0 10 0 0 5