Add to ORKG376-381A method to prepare a concentrated monophasic phenol guanidinium isothiocyanate-based reagent for extraction of RNA from liquid samples is described. The reagent was comparable to a commercial reagent for extraction of genetic material of several RNA viruses as assessed by limit of detection of RNA by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The study showed that the concentrated monophasic reagent could be used in place of a commercial reagent
MicroRNA extraction is an essential procedure when discovering MicroRNA-based biomarkers and approac...
<p>Techniques to isolate the small RNA fraction (<200nt) by column-based methods are commercially av...
<p>EV RNA from 4 mL of plasma or serum was isolated using membrane affinity (spin column), ultracent...
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phen...
Since its introduction, the 'single-step' method has become widely used for isolating total RNA from...
Gene expression studies are a powerful technique to study biological processes, and isolating RNA th...
the residual material in an appropriate buffer for subsequent studies. RNA will be isolated along wi...
Isolation of high-quality functional RNA is prerequisite to facilitate any study related to gene exp...
TRIzol®, TRI Reagent®, and RNAzol® are widely used commercial reagents for the extraction of cellula...
RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are a...
A method for isolation of large, translationally active RNA species is presented. The procedure invo...
Drinking and environmental water samples contain a diverse array of constituents that can interfere ...
For increasedclinicalapplicationsof nucleicacid probes to gene diagnosis, current procedures must be...
Three different methods for RNA preparation using guanidine are presented in this unit--a single-ste...
This procedure is a modification of the original method published by Chirgwin et al. (1). The follow...
MicroRNA extraction is an essential procedure when discovering MicroRNA-based biomarkers and approac...
<p>Techniques to isolate the small RNA fraction (<200nt) by column-based methods are commercially av...
<p>EV RNA from 4 mL of plasma or serum was isolated using membrane affinity (spin column), ultracent...
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phen...
Since its introduction, the 'single-step' method has become widely used for isolating total RNA from...
Gene expression studies are a powerful technique to study biological processes, and isolating RNA th...
the residual material in an appropriate buffer for subsequent studies. RNA will be isolated along wi...
Isolation of high-quality functional RNA is prerequisite to facilitate any study related to gene exp...
TRIzol®, TRI Reagent®, and RNAzol® are widely used commercial reagents for the extraction of cellula...
RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are a...
A method for isolation of large, translationally active RNA species is presented. The procedure invo...
Drinking and environmental water samples contain a diverse array of constituents that can interfere ...
For increasedclinicalapplicationsof nucleicacid probes to gene diagnosis, current procedures must be...
Three different methods for RNA preparation using guanidine are presented in this unit--a single-ste...
This procedure is a modification of the original method published by Chirgwin et al. (1). The follow...
MicroRNA extraction is an essential procedure when discovering MicroRNA-based biomarkers and approac...
<p>Techniques to isolate the small RNA fraction (<200nt) by column-based methods are commercially av...
<p>EV RNA from 4 mL of plasma or serum was isolated using membrane affinity (spin column), ultracent...