Reference

the residual material in an appropriate buffer for subsequent studies. RNA will be isolated along with DNA in thisprocedure.if this is a concern, RNase can be added now or at an earlier step in the procedure. In our experience this procedure is somewhat less efficient than extraction with phenol in terms of recovery of DNA, although the quality of DNA is good for restriction analysis. In in-stances where the quantity of DNA is small, extraction with phenol may be preferable. Although slower, this technique does allow for DNA to be isolated without the use of phenol. We have used this procedure to prepare mammalian ge-nomic DNA for restriction enzyme analysis and have found it to yield high-Mr DNA that is susceptible to restriction enzyme activity. This method provides a significant advantage to any laboratory interested in procedures for isolating DNA with-out the use of noxious chemicals