Three different methods for RNA preparation using guanidine are presented in this unit--a single-step isolation method employing liquid-phase separation to selectively extract total RNA from tissues and cultured cells and two methods that rely on a CsCl step gradient to isolate total RNA
This unit provides two protocols for extraction of RNA from yeast that differ primarily in the metho...
Background: Secondary metabolites are reported to interfere with the isolation of RNA particularly w...
Many experimental techniques related to molecular biology such as the Northern blot analysis, RNA pr...
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phen...
Since its introduction, the 'single-step' method has become widely used for isolating total RNA from...
Most procedures for isolating RNA from eukaryotic cells involve lysing and denaturing cells to liber...
This procedure is a modification of the original method published by Chirgwin et al. (1). The follow...
A method for isolation of large, translationally active RNA species is presented. The procedure invo...
Gene expression studies are a powerful technique to study biological processes, and isolating RNA th...
In a previous report dealing with the guanidine hydrochloride protocol for the extraction of RNA fro...
The protocol described in this unit is a fast and streamlined procedure for preparing total cytoplas...
Most methods for isolation of RNA from yeast require tedious vortexing with glass beads, and give lo...
<p>Techniques to isolate the small RNA fraction (<200nt) by column-based methods are commercially av...
The relevance of RNA in many biological functions has been recognized, broadening the scope of RNA r...
both the expression level of a specific messenger RNA (mRNA) and the expression level of the cognate...
This unit provides two protocols for extraction of RNA from yeast that differ primarily in the metho...
Background: Secondary metabolites are reported to interfere with the isolation of RNA particularly w...
Many experimental techniques related to molecular biology such as the Northern blot analysis, RNA pr...
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phen...
Since its introduction, the 'single-step' method has become widely used for isolating total RNA from...
Most procedures for isolating RNA from eukaryotic cells involve lysing and denaturing cells to liber...
This procedure is a modification of the original method published by Chirgwin et al. (1). The follow...
A method for isolation of large, translationally active RNA species is presented. The procedure invo...
Gene expression studies are a powerful technique to study biological processes, and isolating RNA th...
In a previous report dealing with the guanidine hydrochloride protocol for the extraction of RNA fro...
The protocol described in this unit is a fast and streamlined procedure for preparing total cytoplas...
Most methods for isolation of RNA from yeast require tedious vortexing with glass beads, and give lo...
<p>Techniques to isolate the small RNA fraction (<200nt) by column-based methods are commercially av...
The relevance of RNA in many biological functions has been recognized, broadening the scope of RNA r...
both the expression level of a specific messenger RNA (mRNA) and the expression level of the cognate...
This unit provides two protocols for extraction of RNA from yeast that differ primarily in the metho...
Background: Secondary metabolites are reported to interfere with the isolation of RNA particularly w...
Many experimental techniques related to molecular biology such as the Northern blot analysis, RNA pr...
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