Early Differentiation of Vascular Endothelial Cells During Zebrafish Development

Early differentiation of vascular endothelial cells is indispensable for the proper patterning and lumen formation of blood vessels. The vascular network forms in a reproducible and evolutionarily conserved manner, and their molecular mechanisms of formation have been markedly focused on. However, the differentiation process of vascular progenitors, angioblasts, remains unclear. Here, we report several tools to detect and analyze undifferentiated vascular endothelial cells in zebrafish embryos, including the vascular-specific transgenic lines, inhibitor treatments, and 3D reconstruction technique of electron microscopic serial images.Departmental Bulletin Paper原著2017年度神奈川大学総合理学研究所共同研究助成論文departmental bulletin pape