C7. Perfil de expressão génica por PCR em tempo real em doentes VIH negativos com tuberculose multirresistente

In response to chronic stimulation of M. tuberculosis, multidrug-resistant tuberculosis (MDR-TB) patients up-regulate and downregulate the expression of several genes. Thus, relative quantification of mRNA from genes involved with the immune response could be helpful to elucidate the immunopathology of MDR-TB giving clues to immune modulation.The purpose of this study was to evaluate immunologic gene expression profile associated with MDR-TB.Peripheral blood drawn from 18 MDR-TB patients and 17 healthy control individuals was analysed. Real-time PCR analysis with Taqman® probes was used for relative quantification of mRNAs from genes with important involvement in immune response (cytokines, chemokines, activatory and inhibitory receptors, etc). A panel of primers and probes specific for the following 20 genes was used: IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IFN-g, TGFb, TNF-a, TNF-b, RANTES, MCP1, MIP1b, IP-10, CTLA-4, CD28, CD80 and CD86.In the MDR-TB group, the following cytokine genes were found significantly up-regulated: IFN-g (1.8 logs; p<0.001), IL-10 (2.3 logs; p<0.05), IL-2 (3.0 logs; p<0.001), IL-4 (3.4 logs; p<0.00001) and TNF-b (1.1 logs; p