Comparative effect of TGF-β1 and the TGF-β1RI/ALK5 inhibitor SB431542 on target gene expression in FPTEC, HREC and HK-2.
- Jack S. Lawson (5680364)
- Hui-Hsuan Liu (5621930)
- Harriet M. Syme (831820)
- Robert Purcell (2299924)
- Caroline P. D. Wheeler-Jones (224315)
- Jonathan Elliott (579764)
Publication date
August 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
Effects of TGF-β1 (+/- 5 μM SB431542) on target gene expression was assessed in FPTEC, HREC and HK-2 by RT-qPCR. Target gene mRNA copy number was normalised to GAPDH/RPS7 and is expressed as fold change in relation to control. Data represent three experimental repeats using cells from different cats/batches and were analysed using the one-way ANOVA with Dunnett’s post-hoc analysis. The columns represent the mean normalised mRNA copy number and error bars represent the standard deviation.*PPP<0.001.</p
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