Effect of TGF-β1 on FPTEC, HREC and HK-2 cell morphology.

Representative photomicrographs of FPTEC, HREC and HK-2 after incubation with vehicle and 0.1–10 ng/ml TGF-β1 for 72 h. There was a concentration-dependent loss of the morphology evident in vehicle-treated cells across all three cell types, with cells adopting a hypertrophic, elongated fusiform appearance. These alterations were inhibited by the TGF-β1R1/ALK5 antagonist SB431542 (5 μM). Images are representative of three experiments.