Assessment of the migration capacity of B16.F10 melanoma cells co-cultured with TAMs after different treatments.

(A) B16.F10 cells co-cultured with TAMs before administration (T = 0) and 24 h after administration (T = 24H) of different treatments. Four images per well were taken for each condition (Fig 3A, magnification 100x). (B) The cell migration quantification. The cell migration was estimated by percentages of wound closure compared to the width of the wound at T = 0. Control = untreated cells in co-culture; DMXAA: cell co-culture incubated with 100 μM DMXAA; SIM: cell co-culture incubated with 3.5 μM SIM, SIM+DMXAA: cell co-culture incubated with 3.5 μM SIM administered in combination with 100 μM DMXAA. One-way ANOVA with Bonferroni correction for multiple comparisons was performed to determine the statistical significance (**, PP<0.0001).