<i>In vitro</i> migration and invasion potential of PC-3M-1E8 cells and PC-3M-2B4 cells.

(a) Representative photomicrographs of PC-3M-1E8 cells and PC-3M-2B4 cells are presented at 200 × magnification for migration assay using a microscope (left). Cells migrated were counted from ten fields (2 × (centre + 4 quadrants)). Bar graphs present relative differences in quantity of migrated cells for PC-3M-1E8 cells and PC-3M-2B4 cells (right). (b) Representative photomicrographs of PC-3M-1E8 cells and PC-3M-2B4 cells are presented at 400 × magnification for invasion assay using a microscope (left). Cells invaded were counted from ten fields (2×(centre+4 quadrants)). Bar graphs present relative differences in quantity of invaded cells for PC-3M-1E8 cells and PC-3M-2B4 cells (right) (c) Cell migration evaluated by scratch wound healing assay. Representative images of PC-3M-1E8 cells and PC-3M-2B4 cells migrating into the wounded area at 0, 6, 12 and 24 hr after wound formation are shown (left), and the wound surface areas were measured with Image-Pro Plus software. Line graph presents change in closure of wounds for PC-3M-1E8 cells and PC-3M-2B4 cells (right). The data represents mean ± SD of 3 repeats. There was a significant difference between PC-3M-1E8 cells and PC-3M-2B4 cells (Student’s t-test; *P<0.05).