Migration of pancreatic cancer cells is reduced upon treatment with M344.

Transwell assays were performed to assess the migration of (A) S2-013, (B) BxPC-3, (C) MIA PaCa-2, (D) T3M-4, and (E) CFPAC-1 cells following treatment with 1 μM or 10 μM of M344 (or with 0.1% DMSO, as the vehicle control) for 24 h. For the transwell assays, after treatment the cells were plated into 3 separate 8-μm inserts (1x105 cells/insert) in the presence of 1 μM M344, 10 μM M344, or 0.1% DMSO and incubated for 24 h. Cells were fixed and stained, and photographs of 3 random fields were taken for each of the inserts. The results of counts of the stained, migrated cells were averaged. The graph shows the average numbers of cells that migrated, and each error bar represents the standard error of the mean. The presented graph for the S2-013 cells is representative of the results from 3 separate experiments; the full experimental set of counts for each of the other cell lines was done once. The results of 0.1% DMSO control treatment versus the 1 μM or the 10 μM M344 treatment were compared using Student’s t test. The asterisks indicate the following p values: * p<0.05, ** p< 0.01, *** p<0.001, and **** p<0.0001.