α-Ada and other AP-2 subunits are critical for dendrite pruning in ddaC neurons.

(A–F) Live confocal images of control (A), α-ada3 MARCM (B), α-ada3 MARCM rescue (C), BapΔ1 MARCM (D), BapΔ1 MARCM rescue (E), and AP-2μNN20 MARCM (F) ddaC neurons at WP and 16 h APF. ddaC somas are indicated by red arrowheads. (G) Quantification of percentage of severing defect and fragmentation defect in control and mutant ddaC neurons at 16 h APF. (H) Quantification of total length of unpruned dendrites at 16 h APF. The number of samples (n) in each group is shown on the bars. Error bars represent SEM. Scale bar (A) represents 50 μm. ***p S1 Data. The genotypes can be found in S1 Text. α-Ada, α-Adaptin; α-ada3, α-adaptin3; AP-2μNN20, adaptor protein-2 μ subunitNN20; APF, after puparium formation; BapΔ1, β-adaptinΔ1; FRT, flippase recognition target; GFP, green fluorescent protein; MARCM, mosaic analysis with a repressible cell marker; O/E, overexpression; WP, white prepupal.