Imac plays a crucial role in dendrite pruning of ddaC neurons.

(A–F) Live confocal images of control (A), imac RNAi #1 (B), imac170 MARCM (C), imac170 MARCM rescue (D), non-induced ImacG102E (E), and induced ImacG102E (F) ddaC neurons at WP and 16 h APF. ddaC somas are marked by red arrowheads. (G) Quantification analysis of percentage of severing defect and fragmentation defect in control and imac mutant ddaC neurons at 16 h APF. (H) Quantification of total length of unpruned dendrites at 16 h APF. The number of samples (n) in each group is shown on the bars. Error bars represent SEM. Scale bar (A) represents 50 μm. ***p S1 Data. The genotypes can be found in S1 Text. APF, after puparium formation; imac, immaculate connections; MARCM, mosaic analysis with a repressible cell marker; O/E, overexpression; RNAi, RNA interference; WP, white prepupal.