Proliferation and apoptosis in uteri of <i>Pten</i>^<i>f/f</i><i>Ltf</i>^<i>Cre/+</i> and <i>Pten</i>^<i>f/f</i><i>Pgr</i>^<i>Cre/+</i> mice at 3 months of age.

A, Immunostaining of Ki67 and CK8. The expression of Ki67 show increased proliferation in Ptenf/fLtfCre/+ and Ptenf/fPgrCre/+ epithelium. Note that a line of Ki67+/CK8- cells are under Ki67-/CK8+ epithelial cells. B, Expression of pHH3 in uteri of 3-month-old Ptenf/f, Ptenf/fLtfCre/+ and Ptenf/fPgrCre/+ mice, respectively. C, Immunostaining of p63, CK8, Ki67 and pHH3 on consecutive serial sections. Almost all p63 positive cells are Ki67 positive in Ptenf/fPgrCre/+ uteri. CK8 and p63 positive epithelial cells are separated by dotted lines. D, Immunostaining of Cleaved-caspase-3 in Ptenf/fLtfCre/+ and Ptenf/fPgrCre/+ uteri at 3 months of age. Nuclei are counterstained with Hoechst. Experiments were repeated in three mice, and representative images are presented. Scale bars, 200 μm. le, luminal epithelium; ge, glandular epithelium; s, stroma; myo, myometrium.