Additional file 1: of Growing gold nanostructures for shape-selective cellular uptake

Figure S1. STEM image of Au NRs grown from the solution of (a) low OA concentration (i.e., OA CTAB ~ 10:1) and (b) low ascorbic acid concentration (60 μL of 63 mM). Figure S2. Histograms of differently shaped AuNSs obtained from representative STEM images, showing relative population percentages of (a), (b) nanorods (NRs) (c), (d) tetrahexahedra (THH) (e), (f) nanomakura (NM) (g), (h) bipyramids (BPs), and (i) spheres (SPs). Figure S3. UV-Vis spectra of (a) nanorods (NRs), (b) tetrahexahedra (THH), (c) nanomakura (NM), (d) bipyramids (BPs), and (e) spheres (SPs) after each stage of functionalization. Figure S4. Superimposed images of differently shaped AuNSs after 24-hour co-incubation with glioblastoma-astrocytoma cells. The column to the far left show the halogen images, the middle column shows the cells stained with calcein, while the column to the far right show the superimposed images taken of the cell nuclei (white). Images (a)–(c) show glioblastoma cells incubated with nanorods (NRs), (d)–(f) spheres (SPs), (g)–(i) bipyramids (BPs), (j)–(l) tetrahexahedra (THH), and (m)–(o) nanomakura (NM). Figure S5. Images of nanomakura NSs co-incubated with glioblastoma-astrocytoma cells as a function of time. The column to the far left shows the halogen images; the middle column shows the cells stained with calcein, except image q. The column to the far right shows the superimposed images taken of the cell nuclei (white). Images (a)–(c) were taken after 2 h, (d)–(f) 6 h, (g)–(i) 12 h, (j)–(l) 24 h, (m)–(o): 48 h, and (p)–(r): 72 h of co-incubation. Images (s)–(u) show glioblastoma-astrocytoma cells not incubated with any NSs. (DOCX 2861 kb