Additional file 1: of Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo

Figure S1. a) SEM micrograph of the initial NPR-P. b) Length (L) distribution of the NPRs, average length is 146.2 ± 32.4 nm. c) UV/Vis spectra of a NPR-P solution. Figure S2. a) Agarose gel (0.7%, 100 V, 1 h) of the pegylated NPRs (NPR-P) and the modified ones (NPR-PT, NPR-PTG and NPR-PG). b) ζ-potential measurements of the final NPRs measured in water and using a concentration of 0.04 mg/mL. Figure S3. Fluorescence spectra of NPRs solutions labeled with TAMRA. In black, there is the normalized spectrum for NPR-PT, and in red the normalized spectra for NPR-PTG. Figure S4. UV/Vis spectra of NPRs before (red) and after (black) their incubation in complete cell media during 24 h. Figure S5. (A) Analysis of cell morphology changes by flow cytometry. MiaPaca, HeLa, A549, MEF, B16, MC57G line cells were incubated with four types of nanoparticles (NPR-P, NPR-PG, NPR-PT, NPR-PTG) at four concentrations (25, 50, 100 and 200 μg/mL) for 48 h as indicated in experimental section. After incubation time, cells were analyzed by flow cytometry. A representative experiment is shown at the concentration of 200 μg/mL Note: Ctrl = negative control. Figure S6. Periodic acid-Schiff (PAS) and periodic acid-Schiff diastasa (PAS/D) stain of the liver of mice treated with NPRs. Mice were injected (i.v) with 6 μg/g NPR-PTG and sacrificed after 4 months, the organs were fixed and processed for PAS staining, as indicated in experimental section. Representative images are shown. (DOCX 45107 kb