MOESM1 of Chlorotoxin peptide-functionalized polyethylenimine-entrapped gold nanoparticles for glioma SPECT/CT imaging and radionuclide therapy

Additional file 1: Table S1. The average number of conjugated units on each PEI. Fig. S1. 1H NMR spectra of PEI.NH2-(mPEG) (a), PEI.NH2-(PEG-MAL)-(mPEG) (b), PEI.NH2-(PEG-CTX)-(mPEG) (c), PEI.NH2-HPAO-(PEG-CTX)-(mPEG) (d), PEI.NH2-HPAO-(PEG-MAL)-(mPEG) (e), PEI.NH2-FI-HPAO-(PEG-CTX)-(mPEG) (f), and PEI.NH2-FI-HPAO-(PEG-MAL)-(mPEG) (g) , respectively. UV-vis spectrum and photograph of Au PENPs-CTX dispersed in water (h). Fig. S2. UV-vis spectra of the Au PENPs-CTX dispersed in water at different temperature (a) and pH (b) conditions; Radiochemical purities of the 131I-Au PENPs-CTX and 131I-Au PENPs exposed to PBS at room temperature (c) and FBS at 37 °C (d) for different time periods. Fig. S3. (a) CCK-8 assay of C6 cells treated with the Au PENPs-CTX or Au PENPs at different Au concentrations for 24 h, respectively. (b) CCK-8 assay of C6 cells treated with the 131I-Au PENPs-CTX or 131I-Au PENPs at different 131I concentrations for 24 h, respectively. Fig. S4. Flow cytometric analysis of C6 cells incubated with PBS (a), Au PENPs (b) or Au PENPs-CTX (c) at Au concentration of 5 μM for 4 h, respectively. Part (d) shows the comparison of the binding of Au PENPs and Au PENPs-CTX with C6 cells, and the cells treated with PBS were used as controls. Fig. S5. Confocal microscopy images of C6 cells treated with PBS, Au PENPs or Au PENPs-CTX with Au concentration of 5 μM for 2 h, respectively. Fig. S6. The biodistribution of (a) 131I-Au PENPs-CTX and (b) 131I-Au PENPs, and (c) their relative signal intensities of different organs at 8 h postinjection. Fig. S7. The body weight of C6 tumor-bearing mice after treatments of saline, Au PENPs, Au PENPs-CTX, 131I-Au PENPs, and 131I-Au PENPs-CTX. Saline was used as control. The relative body weight were normalized according to their initial weights (Mean ± SD, n = 5). Fig. S8. Histological changes in the heart, liver, spleen, lung and kidneys of the mice at 2 weeks post-injection of (a) saline, (b) Au PENPs, (c) Au PENPs-CTX, (d) 131I-Au PENPs, and (e) 131I-Au PENPs-CTX. The organ sections were H&E stained and observed under Leica DM IL LED inverted phase contrast microscope at a magnification of 50 × for each sample (the scale bar in each panel indicates 200 μm)