Proximity ligation assay indicates 5-HT<sub>2A</sub>R:5-HT<sub>2C</sub>R interaction within 50 nm.
- Daniel E. Felsing (1567966)
- Noelle C. Anastasio (2063335)
- Joanna M. Miszkiel (5100239)
- Scott R. Gilbertson (1546036)
- John A. Allen (2309494)
- Kathryn A. Cunningham (350338)
Publication date
August 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Schematic of proximity ligation assay (PLA) between 5-HT2CR and 5-HT2AR. (B) Representative 60X confocal photomicrographs of PLA signal from 5-HT2CR and 5-HT2AR heteromeric formation (red puncta) and associated negative controls. PLA was performed using 5-HT2AR (rabbit polyclonal) and 5-HT2CR (mouse monoclonal) primary antibody and oligonucleotide-linked PLA secondary probes [mouse, M(+) and rabbit, R(-)]. Scale bars represent 10 μm. (C) Quantification of puncta from 20X photomicrographs from five independent experiments. *p vs. M(+) and R(-) probes only; ^p vs. 5-HT2AR plus M(+) and R(-) probes; #p vs. 5-HT2CR plus M(+) and R(-) probes.</p
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