Proximity ligation assays for eEF1A and eEF1B.

<p>Panel I: PLA on HeLa cells. A. Negative control with both primary antibodies omitted. B. eEF1A2 antibody only. C. PLA of eEF1A2 and eEF1Bα. D. PLA of eEF1A2 and eEF1Bδ. E. PLA of eEF1A2 and eEF1Bγ. F. PLA of eEF1A2 and TK1 as negative control. Images in the squares are higher magnification of selected areas. Panel II: PLA on NIH-3T3 cells stably expressing V5-tagged eEF1A1. A. Negative control with both primary antibodies omitted. B. V5 antibody only. C. PLA of eEF1Bα and V5. D. PLA of eEF1Bα and TK1. E. PLA of eEF1Bδ and V5. F. PLA of eEF1Bδ and TK1. G. PLA of eEF1Bγ and V5. H. PLA of eEF1Bγ and TK1. Images in the squares are higher magnification of selected areas. Panel III: PLA on NIH-3T3 cells stably expressing V5-tagged eEF1A2. A. Negative control with both primary antibodies omitted. B. V5 antibody only. C. PLA of eEF1Bα and V5. D. PLA of eEF1Bα and TK1. E. PLA of eEF1Bδ and V5. F. PLA of eEF1Bδ and TK1. G. PLA of eEF1Bγ and V5. H. PLA of eEF1Bγ and TK1. Images in the squares are higher magnification of selected areas.