Lung histology.

Representative hematoxylin-eosin stained sections of lung specimens harvested from SO animals treated with vehicle (a) and B. bifidum PRL2010 (b) and from I/R mice pre-treated with vehicle (c) and B. bifidum PRL2010 (d) (scale bar: 100μm). The acute lung injury caused by mesenteric I/R was characterized by diffuse congestion of septae, inter- and intra-alveolar cell infiltration and hemorrhage (c); pre-treatment with B. bifidum PRL2010 attenuated lung injury in all treated mice (d). Damage scoring of lung histological sections, according to Matute-Bello et al. [27], reported in panel e, shows the ability of B. bifidum PRL2010 to alleviate the I/R-induced remote injury (e).