Z-stack of non-transgenic gastrozooid stained with anti-FLAG antibodies. Same polyp as in Additional file 8. Signal from Alexa 647 (white) is shown. (MP4 2059 kb
Abstract Background Hydractinia symbiolongicarpus, a colonial cnidarian, is a tractable model system...
DNA DSB induction in mouse and platypus fibroblasts. Fibroblasts grown on slides were exposed to 5 G...
Figure S2. PCR amplification of sgRNA region for deep-sequencing analysis. (A) Successful transfecti...
Z-stack of Eef1aFLAG-P2A-eGFP gastrozooid stained with anti-FLAG antibodies. Same polyp as in Additi...
Z-stack of non-transgenic gastrozooid. Only DAPI (blue) and eGFP (green) channels are shown. (MP4 48...
Z-stack of stolonal mat and base of gastrozooid from Eef1aFLAG-P2A-eGFP colony. (MP4 15146 kb
Z-stack of Eef1aFLAG-P2A-eGFP gastrozooid. Only DAPI (blue) and eGFP (green) channels are shown. (MP...
Genbank formatted sequence of repair template pUP613 (Eef1a-FLAG-P2A-eGFP). (TXT 9 kb
Genbank formatted sequence of repair template pUP660 (Eef1a-SF-P2A-tdTom) (TXT 10 kb
Figure S1. Pedigree of the colonies used to generate zygotes for microinjection. Figure S2. Effects ...
Alignment of upstream and downstream integration sites from Eef1aSF-P2A-tdTom colonies. Nucleotide a...
Alignment of cloned Eef1a sequences from female colony 295–8. Two PCR amplicons (sized 2.4 kb and 2....
Alignment of upstream and downstream integration sites from Eef1aFLAG-P2A-eGFP colonies. Nucleotide ...
Figure S1. Characterization of dCas9-EGFP knock-in mice. Figure S2. Visualizing telomere dynamics in...
(Movies). Non-targeting siRNA (movie 1) and siPSMA6 (movie 2) were transfected at 25 nM into PANC-1 ...
Abstract Background Hydractinia symbiolongicarpus, a colonial cnidarian, is a tractable model system...
DNA DSB induction in mouse and platypus fibroblasts. Fibroblasts grown on slides were exposed to 5 G...
Figure S2. PCR amplification of sgRNA region for deep-sequencing analysis. (A) Successful transfecti...
Z-stack of Eef1aFLAG-P2A-eGFP gastrozooid stained with anti-FLAG antibodies. Same polyp as in Additi...
Z-stack of non-transgenic gastrozooid. Only DAPI (blue) and eGFP (green) channels are shown. (MP4 48...
Z-stack of stolonal mat and base of gastrozooid from Eef1aFLAG-P2A-eGFP colony. (MP4 15146 kb
Z-stack of Eef1aFLAG-P2A-eGFP gastrozooid. Only DAPI (blue) and eGFP (green) channels are shown. (MP...
Genbank formatted sequence of repair template pUP613 (Eef1a-FLAG-P2A-eGFP). (TXT 9 kb
Genbank formatted sequence of repair template pUP660 (Eef1a-SF-P2A-tdTom) (TXT 10 kb
Figure S1. Pedigree of the colonies used to generate zygotes for microinjection. Figure S2. Effects ...
Alignment of upstream and downstream integration sites from Eef1aSF-P2A-tdTom colonies. Nucleotide a...
Alignment of cloned Eef1a sequences from female colony 295–8. Two PCR amplicons (sized 2.4 kb and 2....
Alignment of upstream and downstream integration sites from Eef1aFLAG-P2A-eGFP colonies. Nucleotide ...
Figure S1. Characterization of dCas9-EGFP knock-in mice. Figure S2. Visualizing telomere dynamics in...
(Movies). Non-targeting siRNA (movie 1) and siPSMA6 (movie 2) were transfected at 25 nM into PANC-1 ...
Abstract Background Hydractinia symbiolongicarpus, a colonial cnidarian, is a tractable model system...
DNA DSB induction in mouse and platypus fibroblasts. Fibroblasts grown on slides were exposed to 5 G...
Figure S2. PCR amplification of sgRNA region for deep-sequencing analysis. (A) Successful transfecti...