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Additional file 1: of Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice

Jinzhi Duan (4874437)
Guangqing Lu (4874434)
Yu Hong (281635)
Qingtao Hu (5948288)
Xueying Mai (5948291)
Jing Guo (102972)
Xiaofang Si (5948294)
Fengchao Wang (506135)
Yu Zhang (12946)

Publication date

November 2018

DOI

10.6084/m9.figshare.7320767.v1

Abstract

Figure S1. Characterization of dCas9-EGFP knock-in mice. Figure S2. Visualizing telomere dynamics in dCas9-EGFP knock-in mouse liver. Figure S3. CRISPR in vivo imaging of major satellite and single genomic locus in X chromosome in dCas9-EGFP knock-in mice. Figure S4. Compare the telomere dynamics in vivo and in vitro. Table S1. Primer sequences. Table S2. Sequences of plasmids. (PDF 1413Â kb

Extracted data

Related items

Additional file 3: Figure S1 and Figure S2. of Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish

Isabel Brocal (436318)
Richard White (138360)
Christopher Dooley (23947)
Samantha Carruthers (63033)
Richard Clark (45325)
Amanda Hall (210816)
Elisabeth Busch-Nentwich (23944)
Derek Stemple (183414)
Ross Kettleborough (3474797)

March 2016

Comparison of induced indels between germline and somatic tissues. (a) Plot shows the same data as F...

Additional file 1: of Considerations for the use of Cre recombinase for conditional gene deletion in the mouse lens

Phuong Lam (6370421)
Stephanie Padula (6370424)
Thanh Hoang (6370427)
Justin Poth (6370430)
Lin Liu (74495)
Chun Liang (72640)
Adam LeFever (6370433)
Lindsay Wallace (5866193)
Ruth Ashery-Padan (389817)
Penny Riggs (6370436)
Jordan Shields (6370439)
Ohad Shaham (389807)
Sheldon Rowan (676027)
Nadean Brown (6370445)
Tom Glaser (243237)
Michael Robinson (2123074)

February 2019

Figure S1. FISH analysis using a probe for CRE labeled in red and a mouse BAC probe (RP24-257H11) la...

Additional file 7: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey Mianné (3407417)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S6. Analysis of the Usp45 project. The figure shows the PCR amplification of the genomic regi...

Additional file 1: of Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

Chenzi Zhang (6163493)
Xiangjun He (6163496)
Yvonne Kwok (6163499)
Feng Wang (44414)
Junyi Xue (1533826)
Hui Zhao (7395)
Kin Suen (6163502)
Chi Wang (131921)
Jianwei Ren (438694)
George Chen (179705)
Paul Lai (6163505)
Jiangchao Li (290762)
Yin Xia (307747)
Andrew Chan (352984)
Wai-Yee Chan (18789)
Bo Feng (141991)

December 2018

Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...

Additional file 3: of In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model

Alba Carreras (6210875)
Luna Pane (6163538)
Roberto Nitsch (6210878)
Katja Madeyski-Bengtson (4452094)
Michelle Porritt (5940611)
Pinar Akcakaya (6210881)
Amir Taheri-Ghahfarokhi (6210884)
Elke Ericson (52165)
Mikael Bjursell (274867)
Marta Perez-Alcazar (6210887)
Frank Seeliger (261686)
Magnus Althage (4347592)
Ralph Knöll (799981)
Ryan Hicks (4579402)
Lorenz Mayr (6210893)
Rosie Perkins (3179886)
Daniel Lindén (483184)
Jan Borén (119843)
Mohammad Bohlooly-Y (217638)
Marcello Maresca (5556467)

January 2019

Figure S2. CRISPR-Cas9 targeting strategy and editing efficiency. (a) Schematic of exon 1 of human P...

Additional file 1: Figure S1. of Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

Van Chu (3428477)
Timm Weber (3428489)
Robin Graf (3428480)
Thomas Sommermann (2406823)
Kerstin Petsch (3428483)
Ulrike Sack (3428492)
Pavel Volchkov (3428486)
Klaus Rajewsky (3247)
Ralf KĂźhn (3428504)

January 2016

CRISPR/Cas9 induced DSBs at the Rosa26 intronic XbaI site in mouse zygotes; Figure S2: Sequence anal...

Additional file 1: of CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling

Haiwei Mou (344158)
Deniz Ozata (4110718)
Jordan Smith (634135)
Ankur Sheel (4110715)
Suet-Yan Kwan (780835)
Soren Hough (6590657)
Alper Kucukural (4026614)
Zachary Kennedy (6590660)
Yueying Cao (4042202)
Wen Xue (698424)

April 2019

Figure S1. Cloning strategy to make donor plasmids. Figure S2. CRISPR-SONIC enables IRES-GFP integra...

Additional file 2: of A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression

Linghe Xi (3513158)
Jens Schmidt (1837222)
Arthur Zaug (3513152)
Dante Ascarrunz (3513155)
Thomas Cech (3513161)

November 2015

A figure showing the TERT copy number analysis for various cell lines generated in this study. (PDF ...

Additional file 1: of Structural rearrangements generate cell-specific, gene-independent CRISPR-Cas9 loss of fitness effects

Emanuel Gonรงalves (5626652)
Fiona Behan (5626649)
Sandra Louzada (188636)
Damien Arnol (1403572)
Euan Stronach (5626676)
Fengtang Yang (45334)
Kosuke Yusa (3175638)
Oliver Stegle (170916)
Francesco Iorio (408438)
Mathew Garnett (408439)

February 2019

Figure S1. CRISPR data overview and quality assessment. Figure S2. Structural rearrangements associa...

Additional file 1 of Small extrachromosomal circular DNA harboring targeted tumor suppressor gene mutations supports intratumor heterogeneity in mouse liver cancer induced by multiplexed CRISPR/Cas9

Tao Guo (126667)
Guo-Qiao Chen (5875136)
Xu-Fan Li (17110322)
Meng Wang (124646)
Kun-Ming Liu (1508101)
Xiao-Ying Yang (2094184)
Si-Cheng Liu (5875133)
Yi-Li Feng (5875118)
Peng-Yuan Liu (60353)
Hui Lin (161703)
An-Yong Xie (13187802)

October 2023

Additional file 1: Table S1. List of target locations with sgRNA sequences, primers for targeted PCR...

Additional file 13: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey Mianné (3407417)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S12. Examples of unexpected point mutations in the F0 animals obtained from the co-injection ...

Additional file 6: of Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

Denise Lanza (5431925)
Angelina Gaspero (5431916)
Isabel Lorenzo (243381)
Lan Liao (625085)
Ping Zheng (21877)
Ying Wang (11406)
Yu Deng (162738)
Chonghui Cheng (5431913)
Chuansheng Zhang (5431922)
John Seavitt (5431928)
Francesco DeMayo (2349946)
Jianming Xu (15308)
Mary Dickinson (4454830)
Arthur Beaudet (544136)
Jason Heaney (5431919)

June 2018

Figure S3. A, B. Examples of mutagenized loxP sites identified upon TA cloning PCR products from lox...

Additional file 14: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey MiannĂŠ (3407429)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S13. Unexpected outcome of CRISPR/Cas9-aided mutagenesis. The figure illustrates an example o...

Additional file 4: of Genome-scale CRISPR-Cas9 knockout screening in gastrointestinal stromal tumor with Imatinib resistance

Jie Cao (353200)
Jianchang Wei (5626712)
Ping Yang (56755)
Tong Zhang (103827)
Zhuanpeng Chen (5626715)
Feng He (47348)
Fang Wei (31236)
Huacui Chen (5626718)
He Hu (122225)
Junbin Zhong (5626721)
Zhi Yang (124104)
Wensong Cai (5626724)
Wanglin Li (5626727)
Qiang Wang (32383)

August 2018

Figure S2. PCR amplification of sgRNA region for deep-sequencing analysis. (A) Successful transfecti...

Additional file 2: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey MiannĂŠ (3407429)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S1. Screening by Sanger sequencing of animals for the generation of a Syt7 conditional allele...

Additional file 3: Figure S1 and Figure S2. of Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish

Isabel Brocal (436318)
Richard White (138360)
Christopher Dooley (23947)
Samantha Carruthers (63033)
Richard Clark (45325)
Amanda Hall (210816)
Elisabeth Busch-Nentwich (23944)
Derek Stemple (183414)
Ross Kettleborough (3474797)

March 2016

Comparison of induced indels between germline and somatic tissues. (a) Plot shows the same data as F...

Additional file 1: of Considerations for the use of Cre recombinase for conditional gene deletion in the mouse lens

Phuong Lam (6370421)
Stephanie Padula (6370424)
Thanh Hoang (6370427)
Justin Poth (6370430)
Lin Liu (74495)
Chun Liang (72640)
Adam LeFever (6370433)
Lindsay Wallace (5866193)
Ruth Ashery-Padan (389817)
Penny Riggs (6370436)
Jordan Shields (6370439)
Ohad Shaham (389807)
Sheldon Rowan (676027)
Nadean Brown (6370445)
Tom Glaser (243237)
Michael Robinson (2123074)

February 2019

Figure S1. FISH analysis using a probe for CRE labeled in red and a mouse BAC probe (RP24-257H11) la...

Additional file 7: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey Mianné (3407417)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S6. Analysis of the Usp45 project. The figure shows the PCR amplification of the genomic regi...

Additional file 1: of Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

Chenzi Zhang (6163493)
Xiangjun He (6163496)
Yvonne Kwok (6163499)
Feng Wang (44414)
Junyi Xue (1533826)
Hui Zhao (7395)
Kin Suen (6163502)
Chi Wang (131921)
Jianwei Ren (438694)
George Chen (179705)
Paul Lai (6163505)
Jiangchao Li (290762)
Yin Xia (307747)
Andrew Chan (352984)
Wai-Yee Chan (18789)
Bo Feng (141991)

December 2018

Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...

Additional file 3: of In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model

Alba Carreras (6210875)
Luna Pane (6163538)
Roberto Nitsch (6210878)
Katja Madeyski-Bengtson (4452094)
Michelle Porritt (5940611)
Pinar Akcakaya (6210881)
Amir Taheri-Ghahfarokhi (6210884)
Elke Ericson (52165)
Mikael Bjursell (274867)
Marta Perez-Alcazar (6210887)
Frank Seeliger (261686)
Magnus Althage (4347592)
Ralph Knöll (799981)
Ryan Hicks (4579402)
Lorenz Mayr (6210893)
Rosie Perkins (3179886)
Daniel Lindén (483184)
Jan Borén (119843)
Mohammad Bohlooly-Y (217638)
Marcello Maresca (5556467)

January 2019

Figure S2. CRISPR-Cas9 targeting strategy and editing efficiency. (a) Schematic of exon 1 of human P...

Additional file 1: Figure S1. of Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

Van Chu (3428477)
Timm Weber (3428489)
Robin Graf (3428480)
Thomas Sommermann (2406823)
Kerstin Petsch (3428483)
Ulrike Sack (3428492)
Pavel Volchkov (3428486)
Klaus Rajewsky (3247)
Ralf KĂźhn (3428504)

January 2016

CRISPR/Cas9 induced DSBs at the Rosa26 intronic XbaI site in mouse zygotes; Figure S2: Sequence anal...

Additional file 1: of CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling

Haiwei Mou (344158)
Deniz Ozata (4110718)
Jordan Smith (634135)
Ankur Sheel (4110715)
Suet-Yan Kwan (780835)
Soren Hough (6590657)
Alper Kucukural (4026614)
Zachary Kennedy (6590660)
Yueying Cao (4042202)
Wen Xue (698424)

April 2019

Figure S1. Cloning strategy to make donor plasmids. Figure S2. CRISPR-SONIC enables IRES-GFP integra...

Additional file 2: of A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression

Linghe Xi (3513158)
Jens Schmidt (1837222)
Arthur Zaug (3513152)
Dante Ascarrunz (3513155)
Thomas Cech (3513161)

November 2015

A figure showing the TERT copy number analysis for various cell lines generated in this study. (PDF ...

Additional file 1: of Structural rearrangements generate cell-specific, gene-independent CRISPR-Cas9 loss of fitness effects

Emanuel Gonรงalves (5626652)
Fiona Behan (5626649)
Sandra Louzada (188636)
Damien Arnol (1403572)
Euan Stronach (5626676)
Fengtang Yang (45334)
Kosuke Yusa (3175638)
Oliver Stegle (170916)
Francesco Iorio (408438)
Mathew Garnett (408439)

February 2019

Figure S1. CRISPR data overview and quality assessment. Figure S2. Structural rearrangements associa...

Additional file 1 of Small extrachromosomal circular DNA harboring targeted tumor suppressor gene mutations supports intratumor heterogeneity in mouse liver cancer induced by multiplexed CRISPR/Cas9

Tao Guo (126667)
Guo-Qiao Chen (5875136)
Xu-Fan Li (17110322)
Meng Wang (124646)
Kun-Ming Liu (1508101)
Xiao-Ying Yang (2094184)
Si-Cheng Liu (5875133)
Yi-Li Feng (5875118)
Peng-Yuan Liu (60353)
Hui Lin (161703)
An-Yong Xie (13187802)

October 2023

Additional file 1: Table S1. List of target locations with sgRNA sequences, primers for targeted PCR...

Additional file 13: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey Mianné (3407417)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S12. Examples of unexpected point mutations in the F0 animals obtained from the co-injection ...

Additional file 6: of Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

Denise Lanza (5431925)
Angelina Gaspero (5431916)
Isabel Lorenzo (243381)
Lan Liao (625085)
Ping Zheng (21877)
Ying Wang (11406)
Yu Deng (162738)
Chonghui Cheng (5431913)
Chuansheng Zhang (5431922)
John Seavitt (5431928)
Francesco DeMayo (2349946)
Jianming Xu (15308)
Mary Dickinson (4454830)
Arthur Beaudet (544136)
Jason Heaney (5431919)

June 2018

Figure S3. A, B. Examples of mutagenized loxP sites identified upon TA cloning PCR products from lox...

Additional file 14: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey MiannĂŠ (3407429)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S13. Unexpected outcome of CRISPR/Cas9-aided mutagenesis. The figure illustrates an example o...

Additional file 4: of Genome-scale CRISPR-Cas9 knockout screening in gastrointestinal stromal tumor with Imatinib resistance

Jie Cao (353200)
Jianchang Wei (5626712)
Ping Yang (56755)
Tong Zhang (103827)
Zhuanpeng Chen (5626715)
Feng He (47348)
Fang Wei (31236)
Huacui Chen (5626718)
He Hu (122225)
Junbin Zhong (5626721)
Zhi Yang (124104)
Wensong Cai (5626724)
Wanglin Li (5626727)
Qiang Wang (32383)

August 2018

Figure S2. PCR amplification of sgRNA region for deep-sequencing analysis. (A) Successful transfecti...

Additional file 2: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey MiannĂŠ (3407429)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S1. Screening by Sanger sequencing of animals for the generation of a Syt7 conditional allele...

Additional file 3: Figure S1 and Figure S2. of Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish

Isabel Brocal (436318)
Richard White (138360)
Christopher Dooley (23947)
Samantha Carruthers (63033)
Richard Clark (45325)
Amanda Hall (210816)
Elisabeth Busch-Nentwich (23944)
Derek Stemple (183414)
Ross Kettleborough (3474797)

March 2016

Comparison of induced indels between germline and somatic tissues. (a) Plot shows the same data as F...

Additional file 1: of Considerations for the use of Cre recombinase for conditional gene deletion in the mouse lens

Phuong Lam (6370421)
Stephanie Padula (6370424)
Thanh Hoang (6370427)
Justin Poth (6370430)
Lin Liu (74495)
Chun Liang (72640)
Adam LeFever (6370433)
Lindsay Wallace (5866193)
Ruth Ashery-Padan (389817)
Penny Riggs (6370436)
Jordan Shields (6370439)
Ohad Shaham (389807)
Sheldon Rowan (676027)
Nadean Brown (6370445)
Tom Glaser (243237)
Michael Robinson (2123074)

February 2019

Figure S1. FISH analysis using a probe for CRE labeled in red and a mouse BAC probe (RP24-257H11) la...

Additional file 7: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey Mianné (3407417)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S6. Analysis of the Usp45 project. The figure shows the PCR amplification of the genomic regi...