Additional file 2: of CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus

Alignment of cloned Eef1a sequences from female colony 295–8. Two PCR amplicons (sized 2.4 kb and 2.0 kb) from the genomic region encoding the 3′ end of Eef1a in colony 295–8 were cloned, sequenced (3 clones each), and aligned. This revealed two alleles, with the major differences being a 52 bp and a 313 bp indel. Differences are shown in red boxes. Sequence features are shown on and above the top sequence of the alignment. Green, Eef1a mRNA as determined by alignments to 291–10 transcripts. Yellow, Eef1a coding sequence. (PDF 4523 kb