nRHO-1* increases transcription levels of <i>dat-1</i>.

a and b Transcription levels of dat-1 or cat-2 in nRHO-1* single and nRHO-1*;dat-1(ok157) double mutants compared to control strains (wild type, dgk-1(sy428)) determined by quantitative PCR. Data presented as mean normalized copy number per reaction ± SEM. Statistical comparisons were performed using one-way ANOVA with Tukey’s multiple test correction. Statistical significance indicated as (***P ≤ 0.001, n.s. = not significant), n = 6, 6, 3, 4 for a and n = 5, 5, 4 for b independent samples for each genotype respectively. c Worm tracking data of body curvature of control animals (wild type, nRHO-1*, nRHO-1*;dat-1(ok157) the same data used in Figs 2B, 3A and 4A), or dgk-1(sy428) animals. Statistical comparisons were performed using one-way ANOVA and presented as mean hip ± SEM with Tukey’s multiple test correction. Statistical significance indicated as (***P ≤ 0.001), n = 70, 32, 14, and 15 animals respectively.d Schematic of the dat-1 gene with the ok157 allele indicated. qPCR primer set for dat-1 within first and second exon shown as arrows.e Worm tracking data of body curvature of control animals (wild type, nRHO-1* the same data used in Figs 2B, 3A, 4A and 6C), unc-31(e928), nRHO-1*;unc-31(e928) and NLP12 OE animals. Statistical comparisons were performed using one-way ANOVA and presented as mean hip ± SEM with Tukey’s multiple test correction. Triangles indicate outliers. Statistical significance indicated as (*P ≤ 0.05, ***P ≤ 0.001), n = 70, 32, 12, 16 and 14 animals respectively.f Transcription levels of nlp-12 in nRHO-1* compared to control (wild type) and NLP12 OE strains determined by quantitative PCR. Data presented as mean normalized copy number per reaction ± SEM. Statistical comparisons were performed using one-way ANOVA with Tukey’s multiple test correction. Statistical significance indicated as (***P ≤ 0.001) n = 6, 6 and 3 independent samples for each genotype respectively. g Transcription levels of dat-1 in control (wild type, nRHO-1* the same data used in Fig 6A) compared to pkc-1(gf), unc-31(e928), nRHO-1*;unc-31(e928) and NLP12 OE strains determined by quantitative PCR. Data presented as mean normalized copy number per reaction ± SEM. Statistical comparisons were performed using one-way ANOVA with Tukey’s multiple test correction. Statistical significance indicated as (*P ≤ 0.05, n.s. = not significant), n = 6 for all apart from n = 3 for NLP12 OE independent samples for each genotype.