A <i>dat-1</i> mutation suppresses nRHO-1* locomotion phenotypes.

a Schematic of the dat-1 gene with alleles ok157 and nz99 indicated. b Worm tracking data of body curvature measurements of control animals (wild type, dat-1(ok157)), nRHO-1* animals, nRHO-1* suppressor strains (nRHO-1*;dat-1(nz99) and nRHO-1*;dat-1(ok157)) and rescue lines. Statistical comparisons were performed using one-way ANOVA and presented as mean hip ± SEM with Tukey’s multiple test correction. Diamonds indicate outliers. Statistical significance indicated as (***P ≤ 0.001, n.s. = not significant), n = 70, 14, 32, 19, 15, 18, and 17 animals respectively.c Dispersal assay as described in materials and methods. Genotypes are color-coded as indicated on the right side of the panel. Hash keys represent data points ≤ 1%. Data presented as the mean percentage of animals on food at indicated time points ± SEM. Statistical comparisons were performed using two-way ANOVA with Tukey’s multiple test correction. Statistical significance indicated as (***P ≤ 0.001, n.s. = not significant), n = 3 experiments (~200 animals per assay).d Aldicarb assay as described in materials and methods. Control strains (wild type, dat-1(ok157)), nRHO-1* animals and nRHO-1*;dat-1(ok157) double mutant assayed on 1mM Aldicarb. Genotypes are color-coded as indicated below the panel. Data presented as mean percentage of paralyzed animals scored every 10 minutes up to 100 min ± SEM, n = 3 (>30 animals per assay).