nRHO-1* increases levels of DAT-1 protein.

a Representative head images of animals expressing dat-1 fused to mCherry under its endogenous promoter (indicated in red), and a general neuronal maker, GFP expressed from the synaptobrevin promoter (indicated in green). Three pairs of dopaminergic head neurons indicated by circles in the left panel. Wild type strain (upper pictures), nRHO-1* animals (lower pictures). Images taken at 100x magnification as described in methods. b Representative head images of heat shock inducible RHO-1* (hsRHO-1*) animals expressing dat-1 fused to mCherry under its endogenous promoter (p.dat-1::mCherry:dat-1) indicated in red, and a general neuronal maker, GFP expressed from the synaptobrevin promoter (p.snb-1::GFP), indicated in green. Three pairs of dopaminergic head neurons indicated by circles in the left panel. No heat shock (middle panel), after heat shock (right panel). Images taken at 100x magnification as described in methods.