Pollen phenotypes, viability and germination analysis in <i>ice1-2</i>.

(A) Alexander staining of the anther, DAPI staining of pollen at tricellular stage, scanning electron microscopy (SEM) of pollen grains from Col-0 and ice1-2. Vn, vegetative nuclei; Sc, sperm cells. (B) FDA (fluorescein diacetate) staining of pollen from Col-0, ice1-2 and c-ice1-2 at flower stage 13. (C) Comparison of viability of pollen from Col-0, ice1-2 and c-ice1-2. SE, n = 5, ** p in vitro germination of pollen from Col-0, c-ice1-2, ice1-2 and selected ice1-2 anthers with obviously open and enlarged stomium (ice1-2-o) at flower stages indicated. The germination rates are listed below photographs. SE, n = 3. (E) Aniline blue-stained pistils of Col-0 flowers at 2 h after pollination with pollen from Col-0, c-ice1-2, ice1-2 and ice1-2-o at flower stages indicated. (F) Scanning electron micrographs of the anther adaxial surface from Col-0, c-ice1-2, ice1-2 and ice1-2-o at flower stages indicated. Arrows indicate the stomium in the ice1-2 anther. St, stomium.