Viability and morphology of mature <i>kinβγ/+</i> pollen grains.

<p>(A–D) Alexander staining revealing the viability of mature pollen of the wild type (A), <i>kinβγ-1/+</i> (B), <i>kinβγ-2/+</i> (C), and <i>kinβγ-2/+ qrt1/-</i> (D). (E–G) DAPI labeling of the nuclei in mature pollen grains of the wild type (E), <i>kinβγ-1/+</i> (F), and <i>kinβγ-2/+</i> (G). (H) Statistical analysis of the trinucleate pollen grains of the wild type (E), <i>kinβγ-1/+</i> (F), and <i>kinβγ-2/+</i> (G). (I–K) SEM analysis of the morphology of mature pollen grains of the wild type (I), <i>kinβγ-1/+</i> (J), and <i>kinβγ-2/+ qrt1/-</i> (K). Arrows indicate the pollen grains with sunken surfaces. (L) Statistical analysis of pollen grains with sunken surfaces. Data were collected from three independent experiments. Asterisks indicate significant difference (Student’s <i>t</i>-test, P<0.01). Bars, 100 μm in (A–C) and 20 μm in (D–G) and (I–K).