Germination analysis of pollen grains <i>in vitro</i> in control and <i>bcmf26a/b</i> lines.

<p>(A and B) Morphological characterization of pollen germination. (A) Pollen tubes from control plants germinate normally. (B) The nonviable pollen grains of the <i>bcmf26a/b</i> lines could not germinate; the germinated pollen tubes of the <i>bcmf26a/b</i> lines exhibited two defective morphologies: pollen wall burst at the beginning of germination and pollen tube tip burst during the elongation process. Images of pollen tubes were obtained at 4 h after germination. Scale bars = 50 μm. (C) Comparison of pollen germination percentage between the pollen of <i>bcmf26a/b</i> and control plants. The percentage of normal pollen tube was only 27.7%–35.1% in the <i>bcmf26a/b</i> lines, but it was ~92.8% in the control. Standard errors are shown.