Additional file 1: of Tracing the biosynthetic origin of limonoids and their functional groups through stable isotope labeling and inhibition in neem tree (Azadirachta indica) cell suspension

This file contains MS data of limonoids, MS/MS data for confirmation of limonoids from cell culture, 13C isotopologue distribution for limonoids, primer information, real time PCR for rate-limiting genes of MVA and MEP pathway. Figure S1. MS/MS confirmation of limonoids identified in cell culture. Figure S2. Variation of intensity of daughter ions of azadirachtin A and its structure fragment relationship. Figure S3. Comparison of isotopologues of limonoids obtained from feeding experiment (with different 13C labeled glucose) with that of the control. Figure S4. Comparison of MS/MS of limonoids obtained from feeding experiment (with different 13C labeled glucose) with that of the control. Figure S5. Relative isotopologue distribution and Heatmap for distribution of isotopologues for specific fragments of 6-deacetylnimbinene. Figure S6. Relative isotopologue distribution and Heatmap for distribution of isotopologues for specific fragments of salannin. Figure S7. Relative expression level of genes of MVA and MEP pathway in different neem tissues. Table S1. MS and MS/MS data for azadirachtin A and its derivatives. Table S2. Primers used in real time PCR analysis. (PDF 2627 kb