MOESM1 of Exploring natural biodiversity to expand access to microbial terpene synthesis

Additional file 1: Table S1. IPK collection from biodiversity. Table S2. Oligonucleotides used in this study for IPK cloning in pCDFDuetTM-1 and/or pRSFDuet™-1. Table S3. List of plasmids used in this study for in vivo screening of IPKs. Figure S1. Absorbance spectrums of carotenoid extracts. Solvent (plain line), E. coli BL21 (DE3) transformed with pACYCDuet™-1 and pETDuet™-1 (round marker) and pACM-EPAG-BPAG and pUCM-IRS (pCarot - diamond marker). Figure S2. HPLC analysis of carotenoids. A Pure neurosporene. B Extract from E. coli cells transformed with ipk-mb. C Pure lycopene. Figure S3. Calibration curves and absorbance spectrums of pure carotenoids. Up-left: HPLC calibration curve of lycopene at 470 nm (X-axis: area of the peak generated at the corresponding retention time; Y-axis: ng of compound injected). Up-right: HPLC calibration curve of neurosporene at 470 nm. Bottom: Absorbance spectrums of pure solutions of lycopene and neurosporene