Additional file 7: of Functional characterization of the cytochrome P450 monooxygenase CYP71AU87 indicates a role in marrubiin biosynthesis in the medicinal plant Marrubium vulgare

Figure S5. Mass spectra and chemical structures for diterpenoids observed in N. benthamiana co-expression assays to test CYP71AU87 substrate promiscuity (see Fig. 6). Transient N. benthamiana co-expression assays were performed by combining MvCYP71AU87 and MvELS with different class II diTPSs, including the maize (Zea mays) ent-CPP synthase ZmAN2 to produce the gibberellin precursor ent-kaurene (compound 9), a LPP synthase from Grindelia robusta (GrLPPS) to produce manoyl oxide (compound 10), and a (+)-CPP synthase from Isodon rubescens (IrTPS3) to form miltiradiene (compound 11). (PDF 322 kb