Quantitation of gene expression differences.

Realtime PCR was performed across two separate experiments (A and B) to measure expression of selected genes determined by transcriptome analysis as upregulated or specifically expressed in 5G3 over 3B5. Also in (A), several genes related to hematopoiesis were shown to be expressed by both 5G3 and 3B5. These included Cxcl12, Csf1, KitL, Spp1 and Wnt5a. Gene expression was quantified in terms of fold change (mean ± SE; n = 2) relative to of Actb (β-actin), a control housekeeping gene. Expression of genes by 5G3 was significantly different to 3B5 (p≥0.05) for all genes except Csf1 and Wnt5a.